map strain atcc 43544 ben a  (ATCC)

Journal: Gut Pathogens

Article Title: RHB-104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of Crohn’s disease associated with Mycobacterium paratuberculosis

doi: 10.1186/s13099-016-0115-3

Figure Lengend Snippet: In - vitro activity of RHB-104 and its individual active ingredients against clinical Mycobacterium strains

Article Snippet: MAP Ben , ATCC 43544 , 0.25 , 0.633 , R , 0.067 , R , 0.3 , R.

Techniques: In Vitro, Activity Assay, Concentration Assay

Journal: Gut Pathogens

Article Title: RHB-104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of Crohn’s disease associated with Mycobacterium paratuberculosis

doi: 10.1186/s13099-016-0115-3

Figure Lengend Snippet: Susceptibility of mycobacterial strains to CLA, CLO, and RIF at their concentrations in the MIC of CLA–CLO–RIF (RHB-104 active ingredients)

Article Snippet: MAP Ben , ATCC 43544 , 0.25 , 0.633 , R , 0.067 , R , 0.3 , R.

Techniques: Comparison

Journal: Gut Pathogens

Article Title: RHB-104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of Crohn’s disease associated with Mycobacterium paratuberculosis

doi: 10.1186/s13099-016-0115-3

Figure Lengend Snippet: Nested PCR analysis of DNA from representative mycobacterial cultures. IS900 -based nested PCR (nPCR) was performed on DNA extracts from MAP and other mycobacterial cultures. PCR products were analyzed on 2 % agarose gel and 298 bp amplified fragment is considered positive for MAP ( lane 20 ). 1 : UCF 5, 2 : UCF 8, 3 : MAP 7, 4 : MAP 8B, 5 : MAP Ben, 6 : MAP Kay, 7 : MAP Linda, 8 : MAP Para, 9 : MAP UCF 3, 10 : MAP UCF 4, 11 : MAP UCF 5, 12 : MAP UCF 7, 13 : MAP UCF 8, 14 : M. avium JF 7, 15 : M. avium JF 8, 16 : MAP Para 18, 17 : M. avium avium , 18 : M. intracellulari LM1A, 19 : M. smegmatis , 20 : M. tuberculosis

Article Snippet: MAP Ben , ATCC 43544 , 0.25 , 0.633 , R , 0.067 , R , 0.3 , R.

Techniques: Nested PCR, Agarose Gel Electrophoresis, Amplification

Journal: BMC Research Notes

Article Title: Purification of native HBHA from Mycobacterium avium subsp . paratuberculosis

doi: 10.1186/1756-0500-6-55

Figure Lengend Snippet: Sequence alignment of different HBHA proteins. ( A ) Multiple sequence alignment was performed using the Clustal W program with the BLOSUM64 matrice allowing gaps (−). * indicate identical residues. : indicate conserved substitutions. ; indicate semi-conserved substitutions. Mtb, M. tuberculosis ; BCG, M. bovis BCG; Mah , M. avium subsp . hominissuis and Map , M. avium subsp . paratuberculosis ; Msmeg, M smegmatis . ( B ) Distribution of charges within the C-terminal domain of HBHA. The isoelectric point of each protein is indicated in brackets. Color code: Polar charged residues HKR (Pink); Polar residues GNQSTY (Green); Polar negative residues DE (Blue) and other residues (Red).

Article Snippet: Mycobacterium avium subsp. paratuberculosis (Map) strain K-10 (ATCC BAA-968) was grown at 37°C in Sauton medium supplemented with 2 mg L -1 of mycobactin J (IDVET, Montpellier, France).

Techniques: Sequencing